黄志伟科研成果

基本信息 新建主栏目 基本信息 名称 黄志伟，哈工大生命科学与技术学院教授、院长，生命科学中心主任。2008年从北京生命科学研究所柴继杰教授结构生物学实验室博士毕业，然后去哈佛大学免疫与感染疾病系 Laurie H. Glimcher教授免疫学实验室做博士后，2012年回国到哈工大建立实验室，研究人适应性免疫的分子机制，以及微生物免疫与新型基因编辑工具的开发。 Dr. Zhiwei Huang, professor and dean of the School of Life Science and Technology and director of HIT Center for Life Sciences at Harbin Institute of Technology. After finishing his PhD thesis from the structural biology lab of Dr. Jijie Chai at National Institute of Biological Sciences in 2008, he then went to the immunological lab of Dr. Laurie H. Glimcher at Harvard University to receive his postdoctoral training. In March 2012, he joined the School and Life Science and Technology at Harbin Institute of Technology as a professor. His research mainly focuses on studying the molecular mechanisms of bacterial and human adaptive immune systems and developing novel genome editing tools. 研究领域 名称 主要研究方向/Research areas 采用整合的研究方法包括生物信息学、病毒学、免疫学、结构生物学以及小鼠遗传学等，从分子、细胞以及个体水平等多个层次以较全视野对适应性免疫系统以及病毒拮抗机制进行研究。研究方向包括： 研究适应性免疫细胞的免疫功能与分子机制。 研究细菌CRISPR-Cas系统以及噬菌体Anti-CRISPR系统的分子机制；设计高效的、特异性的新型基因编辑工具。 研究在生理和病理过程中人微生态系统中病毒、细菌的功能与机制。 解析免疫与感染性疾病发生过程中起关键作用的蛋白（复合物）的分子机制，并利用这些结构信息理性设计小分子药物治疗相关疾病。 We use multi-disciplinary approaches including bioinformatics, single cell sequencing, immunology, structural biology, and mouse models from to study the molecular mechanisms of microbe and human adaptive immune systems, microbial dysbiosis in human diseases, how immune systems interaction with other systems in the body, and developing novel genome editing tools for the treatment of genetic disorders. 代表性文章/Selected Publications ( * Co-first author, # Corresponding author） Xinyu Ma*, Yuwei Zhu*, De Dong*, Yan Chen*, Shubo Wang, Dehui Yang, Zhuo Ma, Anqi Zhang, Fan Zhang, Changyou Guo and Zhiwei Huang# (2022). Cryo-EM structures of two human B cell receptor isotypes. Science. 377, 880-885. (This paper is featured with Perspective, Science. 377, 819-820 (2022)) Yan Chen*, Yuwei Zhu*, Xiang Li*, Wenbo Gao*, Ziqi Zhen, De Dong, Buliao Huang, Zhuo Ma, Anqi Zhang, Xiaocui Song, Yan Ma, Changyou Guo, Fan Zhang, Zhiwei Huang# (2022). Cholesterol inhibits TCR signaling by directly restricting TCR-CD3 core tunnel motility. Molecular Cell. 7;82(7):1278-1287. Fengxia Zhou*, Rui Gan*, Fan Zhang*, Chunyan Ren, Ling Yu and Zhiwei Huang#(2022) PHISDetector: A tool to detect diverse in silico phage-host interaction signals for virome studies. Genomics, Proteomics & Bioinformatics. doi: 10.1016/j.gpb.2022.02.003 Fan Zhang and Zhiwei Huang#(2021) CRISPR and anti-CRISPR: from mechanism to CRISPR toolboxes discovery. Trends in Biochemical Sciences. Hongnan Liu*, Yuwei Zhu*, Zebin Lu, Zhiwei Huang# (2021). Structural basis of Staphylococcus aureus Cas9 inhibition by AcrIIA14. Nucleic Acids Res. 21;49(11):6587-6595. Xianwen Ren*, Wen Wen*, Xiaoying Fan*, Wenhong Hou*, Bin Su*, Pengfei Cai*, Jiesheng Li*, Yang Liu*, Fei Tang*, Fan Zhang*, Yu Yang*……Zhiwei Huang#, Jin-Xin Bei#, Lai Wei#, Xiu-Wu Bian#, Xindong Liu#, Tao Cheng#, Xiangpan Li#, Pingsen Zhao#, Fu-Sheng Wang#, Hongyang Wang#, Bing Su#, Zheng Zhang#, Kun Qu#, Xiaoqun Wang#, Jiekai Chen#, Ronghua Jin#, Zemin Zhang# (2021). COVID-19 immune features revealed by a large-scale single-cell transcriptome atlas. Cell. 184(7):1895-1913. Shengli Ma*#, Fan Zhang*, Fengxia Zhou*, Hui Li*, Wenyu Ge, Rui Gan, Huan Nie, Biao Li, Yindong Wang, Meng Wu, Duo Li, Dongmei Wang, Zheng Wang, and Yuhong You, Zhiwei Huang# (2021). Metagenomic analysis reveals throat microbiota alterations in patients with COVID-19. Signal Transduction Targeted Therapy. 6, 191. Fan Zhang*, Rui Gan*, Ziqi Zhen*, Xiaoli Hu*, Xiang Li, Fengxia Zhou, Ying Liu, Chuangeng Chen, Shuangyu Xie, Bailing Zhang, Xiaoke Wu, Zhiwei Huang# (2020). Adaptive immune responses to SARS-CoV-2 infection in severe versus mild individuals. Signal Transduction Targeted Therapy. 5(1):156. Kaiming Zhang*, Shuo Wang*, Sahnshan Li*, Yuwei Zhu*, Grigore D. Pintilie, Tung-Chung Mou, Michael F. Schmid, Zhiwei Huang# & Wah Chiu# (2020). Inhibition mechanisms of AcrF9, AcrF8, and AcrF6 against type I-F CRISPR-Cas. PNAS. 117(13):7176-7182. De Dong*, Lvqin Zheng*, Jianquan Lin*, Bailing Zhang, Yuwei Zhu, Ningning Li, Shuangyu Xie, Yuhang Wang, Ning Gao#, Zhiwei Huang# (2019). Structural basis of assembly of the human T cell receptor-CD3 complex. Nature. 573(7775):546-552. (This paper is featured with News & Views, Nature. 573, 502-504 (2019)) Liyong Dong*, Xiaoyu Guan*, Ningning Li*, Fan Zhang, Yuwei Zhu, Kuan Ren, Ling Yu, Fengxia Zhou, Zhifu Han, Ning Gao & Zhiwei Huang# (2019). An anti-CRISPR protein disables type V Cas12a by acetylation. Nature Structural & Molecular Biology. 26(4):308-314. (This paper is featured with News & Views, Nature Structural & Molecular Biology. 26(4):250-251 (2019)) Minghui Guo*, Kaiming Zhang*, Yuwei Zhu, Grigore D. Pintilie, Xiaoyu Guan, Shanshan Li, Michael F. Schmid, Zhuo Ma, Wah Chiu# & Zhiwei Huang# (2019). Coupling of ssRNA Cleavage with DNase Activity in Type III-A CRISPR-Csm Revealed by Cryo-EM and Biochemistry. Cell Research. 29(4):305-312. Minghui Guo*, Kuan Ren*, Yuwei Zhu*, Ziyun Tang, Yuhang Wang, Bailing Zhang & Zhiwei Huang# (2019). Structural insights into a high fidelity variant of SpCas9. Cell Research. 29,183-192. Yuwei Zhu, Zhiwei Huang# (2018). Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools. National Science Review. doi: 10.1093/nsr/nwy150 Fan Zhang#, Shijia Zhao, Chunyan Ren, Yuwei Zhu, Haibin Zhou, Yongkui Lai, Fengxia Zhou, Yuqiang Jia, Kangjie Zheng & Zhiwei Huang# (2018). CRISPRminer is a knowledge base for exploring CRISPR-Cas systems in microbe and phage interactions. Communications Biology. 31;1:180. De Dong*, Minghui Guo*, Sihan Wang*, Yuwei Zhu, Shuo Wang,Zhi Xiong, Jianzheng Yang, Zengliang Xu and Zhiwei Huang# (2017). Structural basis of CRISPR-SpyCas9 inhibition by an anti-CRISPR protein. Nature. 546(7658):436-439. Dan Wu*, Xiaoyu Guan*, Yuwei Zhu*, Kuan Ren, Zhiwei Huang# (2017). Structural basis of stringent PAM recognition by CRISPR-C2c1 in complex with sgRNA. Cell Research. 27(5):705-708. Pu Gao, Hui Yang, Kanagalaghatta R Rajashankar, Zhiwei Huang and Dinshaw J Patel (2016). Type V CRISPR-Cas Cpf1 endonuclease employs a unique mechanism for crRNA-mediated target DNA recognition. Cell Research. 26(8):901-13. De Dong*, Kuan Ren*, Xiaolin Qiu*, Jianlin Zheng, Minghui Guo, Xiaoyu Guan, Hongnan Liu, Ningning Li, Bailing Zhang, Daijun Yang, Chuang Ma, Shuo Wang, Dan Wu, Yunfeng Ma, Shilong Fan, Jiawei Wang, Ning Gao and Zhiwei Huang# (2016). Crystal structure of CRISPR-Cpf1 in complex with CRISPR RNA (crRNA). Nature. 532(7600):522-6. Yingying Guo*, Liyong Dong*, Xiaolin Qiu*, Yishu Wang, Bailing Zhang, Hongnan Liu, You Yu, Yi Zang, Maojun Yang and Zhiwei Huang# (2014). Structural basis for hijacking CBF-β and CUL5 E3 ligase complex by HIV-1 Vif. Nature. 505(7482):229-33. (This paper is featured with News & Views, Nature. 505, 167-168, highlighted in Nature Structural & Molecular Biology, 21, 117 (2014)) Jae-Hyuck Shim, Matthew B. Greenblatt, Weiguo Zou, Zhiwei Huang, Marc N. Wein, Nicholas Brady, Dorothy Hu, Jean Charron, Heather R. Brodkin, Gregory A. Petsko, Dennis Zaller, Bo Zhai, Steven Gygi, Laurie H. Glimcher and Dallas C. Jones (2013). Schnurri-3 regulates ERK downstream of WNT signaling in osteoblasts. J Clin Invest. 123(9):4010-22. Zehan Hu, Chuangye Yan, Peiyuan Liu, Zhiwei Huang, Rui Ma, Chenlu Zhang, Ruiyong Wang, Yueteng Zhang, Fabio Martinon, Di Miao, Haiteng Deng, Jiawei Wang, Junbiao Chang, Jijie Chai (2013). Crystal structure of NLRC4 reveals its autoinhibition mechanism. Science. 341(6142):172-5. Weiguo Zou, Xi Chen, Jae Shim, Zhiwei Huang, Nicholas Brady, Dorothy Hu, Rebecca Drapp, Kirsten Sigrist, Laurie H. Glimcher, Dallas Jones (2011). The E3 ubiquitin ligase Wwp2 regulates craniofacial development through monoubiquitination of Goosecoid. Nature Cell Biology. 13(1):59-65. Zhiwei Huang*, Sarah E. Sutton*, Adam J. Wallenfang, Robert C. Orchard, Xiaojing Wu, Yingcai Feng, Jijie Chai and Neal M. Alto (2009). Structural insights into host GTPase isoform selection by a family of bacterial GEF mimics. Nature Structural & Molecular Biology. 16(8):853-60. (This publication was selected as cover story, and high-lighted by Nature China） Zhiwei Huang, Yingcai Feng, Ding Chen, Xiaojing Wu, Xiaojun Wang, Xingguo Xiao, Wenhui Li, Niu Huang, Lichuan Gu, Guangming Zhong and Jijie Chai (2008). Structural basis for activation and inhibition of the secreted chlamydia protease CPAF. Cell Host & Microbe. 4(6):529-42. (This publication was selected as a research “highlight” by Nature China） Maikke B. Ohlson, Zhiwei Huang, Neal M. Alto, Jack E. Dixon, Jijie Chai and Samuel I. Miller (2008). Structure and Function of Salmonella SifA Indicate that Its Interactions with SKIP, SseJ, and RhoA Family GTPases Induce Endosomal Tubulation. Cell Host & Microbe. 4(5):434-46. 招聘、研究生招生需求 名称 招聘副研究员、博士后、技术员、博士生： 欢迎具有适应性免疫学（免疫疾病或病毒感染动物模型）、结构生物学（冷冻电镜、大分子晶体学）、噬菌体生物学、在体基因编辑、CRISPR筛选、计算生物学（单细胞多组学数据分析、机器学习、化合物虚拟筛选）、动物疾病模型（眼底病、血液病）研究背景的人员申请。请申请者将一份CV、以及三位推荐人的联系方式发至 huangzhiwei@hit.edu.cn 实验室链接：http://huanglab.hit.edu.cn/ 荣誉与项目 名称 科学探索奖，2020 国家高层次人才计划，2018 国家杰出青年科学基金，2018 谈家桢生命科学创新奖，2018 中国青年科技奖，2018 国家高层次青年人才计划，2015 国家优秀青年科学基金，2014 黑龙江省杰出青年科学基金，2014