欧志敏科研成果

个人简介 浙江工业大学药学院教授，博士生导师，硕士生导师。2003年6月， 毕业于浙江大学生物化工专业，获工学博士学位。2003-今，浙江工业大学，药学院，生物制药系，微生物与生化药学方向，教学科研。2013.10-2014.10 美国伦斯勒理工生物催化与代谢工程方向访问学者。主持参与多项国家级和省部级科研项目，发表文章100余篇。授权专利20余项，专利转让6项。浙江省药学会生物制药专委会委员。主要研究方向：生物制药、手性药物的生物合成，生物催化与转化技术、体外多酶级联技术、合成生物学、分子生物学。 承担课程：本科生：“制药设备与车间设计”和“制药工艺课程设计”。研究生：“生化反应工程与反应器”和“手性药物的生物合成（双语课程）”。主持项目： 1. 基于辅酶再生的纳米高分子-双酶结合体的理性设计及在油水两相中增效不对称还原反应的机制，国家自然科学基金面上项目。2.氧化还原酶的应用，浙江九洲药物科技有限公司，重大横向项目。3.界面自组装羰基还原酶和辅酶的制备及其在油-水界面催化手性醇的合成，浙江省自然科学基金。4.手性醇类药物中间体的生物法清洁合成，浙江九洲药物科技有限公司，重大横向项目。5.酶活指纹谱法高通量筛选不对称还原芳香族β-羰基酯的高效抗体酶，浙江省自然科学基金。6.抗抑郁药手性中间体β-羟基苯丙酸乙酯的生物合成新技术，浙江省科技厅科技计划项目。7.β-葡萄糖苷酶高产菌株发酵法炮制牛蒡子的研究，浙江省中医药管理局项目。8.《生物制药设备》校级优秀课程。9.校级本科核心课程《制药设备与车间设计》、《制药工艺课程设计》。10.校级研究生教改项目《手性药物生物合成》双语课程。11.校级研究生实践教学改革项目。12.校级研究生在线课程建设项目《手性药物的生物合成》。代表论文：(1)Mei Liu, Nana Wang, Lixia Deng, Bo Liu, Yanni Wang, Qingyu Zhang, Lan Tang, Qingqing Zhou, Jianhong Pan, Zhimin Ou*. Dual enzyme self-assembly cluster for high efficient asymmetric reduction of ethyl 6-oxo-8-chlorooctanoate. Biochemical Engineering Journal, 2024, 207:109319(2)Yuan Lu, Jinmei Wang, Pengpeng Cheng, Chuyue Zhang, Jinghua Li, Zhimin Ou*. Efficient biosynthesis of (R)− 1-[3,5-bis(trifluoromethyl)phenyl] ethanamine with self-assembled dual-enzyme clusters. Process Biochemistry, 2023,134:151–164(3)Muhammad Naeem, Abdul Nasir, Mengjun Fang, Zhimin Ou*, Peijie Shen, Zhenjun Si, Zhinan Xu*. Characterization and rational engineering of Butyrivibrio crossotus argonaute for improved cleavage activity.Biochemical  Engineering Journal,2024,202:109113(4)Yuan Lu, Jinmei Wang, Haobo Xu, Chuyue Zhang, Pengpeng Cheng, Lihua Du, Lan Tang, Jinghua Li, Zhimin Ou*.  Efficient Synthesis of Key Chiral Intermediate in Painkillers (R)-1-[3,5-Bis(trifluoromethyl)phenyl]ethanamine by Bienzyme Cascade System with R-ω-T ransaminase and Alcohol Dehydrogenase Functions. Molecules, 2022, 27(21),7331-7348. (5)Jinmei Wang, Yuan Lu, Pengpeng Cheng, Chuyue Zhang, Lan Tang, Lihua Du, Jinghua Li, Zhimin Ou*.  Construction of Bi-Enzyme Self-Assembly Clusters Based on SpyCatcher/SpyTag for the Efficient Biosynthesis of (R)-Ethyl 2-hydroxy-4-phenylbutyrate. Biomolecules, 2023,13,91.DOI：10.3390/biom13010091. (6)Xin-Yuan Liu, Yi-Li Chen, Guo-Jian Liu, Xiang-Nan Deng, Yue Cui, Jie Tan, Xing-Chen Dong, Hua-Ying Li, Gan-Jun Chen, Zhimin Ou*, Chun-He Wang. Development of a variant of dinutuximab with enhanced antitumor efficacy and reduced induction of neuropathic pain. FEBS Open Bio, 2022,12(9):1664-1656. (7) Hongqian Dai; Yuan Lu; Hanbing Shi; Lan Tang; Xingyuan Sun; Zhimin Ou*. Efficient enantiomer selective acetylation of 1-methyl-3-phenylpropylamine by Fe3O4-APTES-CS2-lipase magnetic nano particles in an alternating magnetic field, Biocatalysis and Biotransformation, 2021, 10.1080/102 42422.202(10.1080/10242422.202)   (8)Yuan Lu; Hongqian Dai; Pengpeng Cheng; Hanbing Shi; Lan Tang; Xingyuan Sun;  Zhimin Ou*. Regenerated coenzyme-based preparation of bienzyme-polymer nanoconjugates and their applications f or the synthesis of ethyl (R)-2-hydroxy-4-phenylbutyrate, Korean J. Chem. Eng., 2021.春季, 38(5): 1066-1077 (9)Yuan Lu, Hongqian Dai, Hanbing Shi, Lan Tang, Xingyuan Sun, Zhimin Ou*. Synthesis of ethyl (R)-4-chloro-3-hydroxybutyrate by immobilized cells using amino acid-modified magnetic nanoparticles. Process Biochemistry,2020,99:9–20.  (10)Zhimin Ou*; Jiaying Pan; Lan Tang; Hanbing Shi ; Continuous enantiomer-selective acylation reaction of 1-phenylethanamine in a magnetic fluidized bed reactor system (MFBRS), Journal of Che mical Technology and Biotechnology, 2019, 94: 1951-1957 (11)Zhimin Ou*; Jiahui Xu; Lihua Du; Lan Tang; Yangping Niu; Jian Cui ; Preparation of Interfa ce-Assembled Carbonyl Reductase and Its Application in the Synthesis of S-Licarbazepine in Toluen e/Tris-HCl Buffer Biphasic System, Journal of Microbiology and Biotechnology, 2018, 28(4): 613-621   (12)Jiaying Pan; Zhimin Ou*; Lan Tang; Hanbing Shi ; Enhancement of catalytic activity of lipa se-immobilized Fe 3 O 4 -chitosan microsphere for enantioselective acetylation of racemic 1-pheny lethylamine, Korean Journal of Chemical Engineering, 2019, 36(5): 729-739    (13)Zhimin Ou*, Han-bing Shi, Xing-yuan Sun, Wen-he Shen. Synthesis of SLicarbazepine by asymmetric reduction of oxcarbazepine with Saccharomyces cerevisiae CGMCC No. 2266.  Journal of Molecular Catalysis B: Enzymatic, 2011,72(3-4):294-297. (14) Zhimin Ou*, Xiaoyan Chen, Guoqing Ying, Hanbing Shi, Xingyuan Sun. Continuous preparation of (S)-3-hydroxy-3-phenylpropionate by asymmetric reduction of 3-oxo-3-phenyl-propionic acid ethyl ester with Saccharomyces cerevisiae CGMCC No. 2266 in a membrane reactor.  Biotechnology and Bioprocess Engineering, 2011,16(2):320-326. (15)Z. Ou*, G. Yang. Asymmetric reduction of  Carbonyl phenylpropionate by undifferentiated cells of white turnip in phosphate buffer/organic solvent. New Biotechnology,2009,25:s140. (16) Zhimin Ou*, Jianping Wu, Lirong Yang, Peilin Cen. Asymmetric reduction of chloroacetophenones to produce chiral alcohols with microorganisms. Korean Journal of Chemical Engineering,2008,25(1):124-128. (17) OU Zhimin*, GUO Fangyuan, YANG Gensheng, YAO Shanjing. Enhancement of arctigenin content by processing of Fructus Arctii via solid-state fermentation coupled with poach.Chinese Journal of Catalysis,2009,30(1):48-52. (18)OU Zhimin*, YANG Gensheng, GUO Fangyuan. Preparation of (S)-(-)-β-hydroxy-benzenepro-panoic acid ethyl ester by asymmetric reduction of β-Carbonyl phenylpropionate with immobilized CGMCC No.2266. Huagong Xuebao / CIES Journal, 2009,60(2):421-427.  教学与课程 育人成果 科研项目 科研成果 社会服务 内容来自集群智慧云企服 软件产品登记测试全国受理